p70S6K (S6K1), GST-tag Recombinant

Catalog #

40062

$375 *

Size: 20 µg

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Description

Human p70S6Kb1, also known as ribosomal protein S6 kinase, 70 kDa, polypeptide 1 (RPS6KB1), GenBank Accession No. NM_003161, amino acid 1-421 with N-terminal GST tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 1 time.

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Synonyms

RPS6KB1, p70S6K, Ribosomal S6 kinase, S6K, STK14A

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Product Data Gallery

P70S6K Specific Activity

10% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.

74 kDa

Amino Acids

1–421

Specific Activity

2 pmol/min/µg

Genbank #

NM_003161

UniProt #

P23443

Tag(s)

N-terminal GST-tag

Background

p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).

References

1. Di Paolo S. et al., Am. J. Kidney Dis. 49 (3), 462-470 (2007).
2. Fang C.X. et al., J. Exp. Neurol. 203 (1), 196-204 (2007).

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (SER/THR 7, Invitrogen) at room temperature for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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p70S6K (S6K1), GST-tag Recombinant

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