p70S6K (S6K1) Kinase Assay Kit
The P70S6K (S6K1) Kinase Assay Kit is designed to measure P70S6K (S6K1) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
- ADP-Glo® Kinase Assay (Promega #V6930)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40062 | P70S6K (S6K1), GST-Tag* | 15 µg | -80°C |
| 79334 | Kinase Buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 50 µl | -20°C |
| S6K Substrate (1 mg/ml) | 500 µl | -20°C | |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
p70S6K is responsible for the phosphorylation of the 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. In intact cells p70S6K is activated by serum and this activation is inhibited by wortmannin and rapamycin. p70S6k activity is cell cycle-dependent and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylation of serine residues in the putative auto-inhibitory pseudo-substrate domain, of threonine 389 phosphorylated by mTOR, and of threonine 229 phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
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