EPHA4 Kinase Assay Kit
The EPHA4 Kinase Assay Kit is designed to measure EPHA4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
- ADP-Glo® Kinase Assay (Promega #V6930)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40193 | EPHA4, GST-Tag* | 5 µg | -80°C |
| 79334 | Kinase Assay Buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 50 µl | -20°C |
| 40217 | PTK Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) | 50 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
EPH receptor A4 (EPHA4), belongs to the ephrin receptor subfamily of protein-tyrosine kinases which have been implicated in mediating developmental events, particularly in the nervous system. The EPHA4 ligand ephrin-A3 is localized to the astrocytic processes that envelop the spine. Activation of EPHA4 by ephrin-A3 induces spinal retraction, reduces spine density, and inhibits the interaction distorted spine shape and organization. EPHA4-null mice possess defects in the corticospinal tract and anterior commissure indicating a model in which an ephrin ligand on the axons senses EPHA4 on spinal cord cells surrounding the corticospinal tract.
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