FGFR2 Assay Kit

Catalog #

79804

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FGFR2 Assay Kit is designed to measure FGFR2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against FGFR2? Check out our Kinase Screening Services.

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Synonyms

FGFR2, Fibroblast Growth Factor Receptor 2, BEK, Keratinocyte Growth Factor Receptor, KGFR, JWS, CD332, FGFR-2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The FGFR2 Assay Kit comes in a convenient 96-well format, with enough purified recombinant FGFR2, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 0.5 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40211	FGFR2, GST-Tag	5 µg	-80°C	Avoid mulitple freeze/thaw cylces!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
40217	Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P21802

Background

FGFR2 (Fibroblast Growth Factor Receptor 2) is a member of a family of tyrosine kinases involved in many pathways that play a significant role in cancer. Amplification or activation of FGFR2 has been reported in breast and gastric cancers, while FGFR2 mutations have been observed in endometrial and breast cancers. Mutations in FGFR2 are also associated with bone development disorders including Pfeiffer Syndrome and Crouzon Syndrome.

References

1. Rutland, P., et al. 1995. "Identical mutations in the FGFR2 gene cause both Pfeiffer and Crouzon syndrome phenotypes." Nature Genetics 9(2): 173.
2. Xie, L., et al. 2013. "FGFR2 gene amplification in gastric cancer predicts sensitivity to the selective FGFR inhibitor AZD4547." Clinical Cancer Research 9(2): 2572-2583.

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only