FGFR1(FLT2), GST-tag Recombinant

Catalog #

40210

$410 *

Size: 10 µg

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Description

Recombinant human FGFR1 (Fibroblast Growth Factor Receptor 1), encompassing amino acids 399-822 (end) and containing the kinase domain. This construct contains an N-terminal GST-tag. This recombinant protein was affinity purified and is kinase active.

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Synonyms

CEK, FLG, FLT2, KAL2, BFGFR, CD331, FGFBR, HBGFR, N-SAM, C-FGR, FGFR-1, FLT-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

FGFR1 (GST-399-822)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol

73 kDa

Amino Acids

399-822

Specific Activity

62 pmol/min/µg

Genbank #

NM_023110

UniProt #

P11362

Tag(s)

N-terminal GST-tag

References

1. Xu, X. et al: Fibroblast growth factor receptors (FGFRs) and their roles in limb development. Cell Tissue Res. 1999 Apr;296(1):33-43.
2. Groth, C. et al: The structure and function of vertebrate fibroblast growth factor receptor 1. Int J Dev Biol. 2002;46(4):393-400.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

FGFR1 (FLT2) activity was measured by using a Poly (4:1 Glut, Tyr) Peptide Substrate, diluted in 25 mM Tris-HCl pH 7.5 to a final concentration of 1 mg/ml, in the ADP Glo™ Kinase Assay Kit (Promega #V9101). FGFR1 (FLT2) was diluted at different concentrations in 1x Kinase Assay Buffer (40 mM Tris-HCl, pH 7.4, 20 mM MgCl₂, 2.5 mM MnCl₂, 0.1 mg/ml BSA and 50 µM DTT). A Substrate/ATP mix was prepared by adding 1 µl of 10 mM ATP, 78 µl of 5x Kinase Assay Buffer, 80 µl of 1 mg/ml substrate and 1 µl of 1 M of MnCl₂. The reaction was initiated by the addition of 3 µl of diluted FGFR1 (FTL2) to 2 µl of Substrate/ATP Mix.

After a 40-minute incubation at Room Temperature (RT), the reaction was terminated by addition of ADP-Glo™ Reagent, followed by a subsequent 40-minute incubation at RT. Kinase Detection Reagent was added, and the reaction was incubated for another 30 minutes at RT. Detection of luminescence was measured using the Luminescence Module Protocol on GloMax®-Multi Microplate Multimode Reader. The Specific Activity was calculated as follows: (Corrected activity, RLU) / [(Specific activity from ADP in RLU/pmol) * (Reaction time in min) *(Enzyme amount in μg or mg)]. Corrected RLU was calculated by subtracting the blank value from all the values. The blank was determined from a “no enzyme” sample by replacing the enzyme solution with an equal volume of 1x Kinase Assay Buffer.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40210 Lot 240415

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FGFR1(FLT2), GST-tag Recombinant

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