p70S6Kb, GST-tag Recombinant

Catalog #

40146

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human p70S6Kb, also known as RPS6KB2, STK14B, KLS, P70-beta, or SRK, GenBank Accession No. BC_000094, fulllength with an N-terminal GST tag and expressed in a baculovirus-infected Sf9 insect cell expression system. MW = 85 kDa.

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Synonyms

RPS6KB2, STK14B, KLS, P70-beta, P70-beta-1, P70-beta-2, S6K2, p70(S6K)-beta, S6K-beta2, SRK

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

85 kDa

Amino Acids

full length

Specific Activity

107 pmol/min/µg

Genbank #

BC000094

UniProt #

Q9UBS0

Tag(s)

N-terminal GST-tag

Background

p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. p70S6Kb contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. PI3 kinase pathway and mTOR are involved in the activation of p70S6Kb but other pathways can also activate this target protein.

References

1. Gout, I. et al: Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region. J. Biol. Chem. 273: 30061-30064, 1998.
2. Saitoh, M. et al: Cloning and characterization of p70(S6K-beta) defines a novel family of p70 S6 kinases. Biochem. Biophys. Res. Commun. 253: 470-476, 1998.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate p70S6K with 0.2 mg/ml RSK-sub peptide substrate and 50 ?M [³³P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40146_Lot141210.pdf

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p70S6Kb, GST-tag Recombinant

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