NLRP3 (delPYD) / NEK7 Complex Recombinant

Catalog #

100477

$565 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Complex of NLRP3 and NEK7 proteins. The two proteins were co-expressed and affinity purified.

Recombinant human NLRP3 (NLR family pyrin domain containing 3), encompassing amino acids 134-1036 (end). The construct does not contain the pyrin domain (also called DAPIN domain or PYD), which is involved in PYCARD binding. It contains an N-terminal FLAG-tag followed by an Avi-Tag™.

Recombinant human NEK7 (NIMA related kinase 7), encompassing amino acids 2-302 (end). This construct contains an N-terminal His-tag (7xHis).

●

Synonyms

NACHT, LRR and PYD domains-containing protein 3, AII, AVP, FCU, MWS, FCAS, KEFH, CIAS1, FCAS1, NALP3, C1orf7, Caterpillar protein 1.1, CLR1.1, DFNA34, PYPAF1, AGTAVPRL, Cryopyrin, NIMA related kinase 7, Never in mitosis A-related kinase 7

●

Product Data Gallery

4-20% SDS-Page Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Construct

NLRP3 (FLAG-Pre-134-1036(end)) / NEK7 (His-Pre-2-302(end))

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous Buffer Solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 μg/ml FLAG peptide, 3 mM DTT and 20% glycerol.

NLRP3: 105 kDa; NEK7: 37 kDa

Amino Acids

NLRP3: 134-1036(end); NEK7: 2-302(end)

Genbank #

NLRP3: NM_004895; NEK7: NM_133494

UniProt #

NLRP3: Q96P20; NEK7: Q8TDX7

Tag(s)

NLRP3: N-terminal FLAG-tag; NEK7: N-terminal His-tag

References

1. He, Y., et al. Nature, 2016 Feb; 530: 354-357.
2. He, Y., et al. Trends in Biochem. Sci. 2016 Dec; 41(12):1012-1021.

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

100477 Lot 200506

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

NLRP3 (delPYD) / NEK7 Complex Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY