NEK9, GST-tag Recombinant

Catalog #

40142

$375 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a 1-345, 732-end (a.a. 1-979 with deleted fragment a.a. 346-731), with N-terminal GST-tag, MW =92 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

NEK9, NIMA-related kinase 9, Never In Mitosis-Related Kinase 9, NERCC

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥38%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.

92 kDa

Amino Acids

1–979 (end), delta 346–731

Specific Activity

147 pmol/min/µg

Genbank #

NM_033116

UniProt #

Q8TD19

Tag(s)

N-terminal GST-tag

References

1. Roig, J., et al. Genes Dev. 16: 1640-1658 (2002).
2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml MBP for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40142_Lot111129-5.pdf

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