NCAM1/CD56 Positive Cell Isolation Kit
The NCAM1/CD56 Positive Cell Isolation Kit is designed to magnetically separate NCAM1/CD56-expressing cells from a complex immune cell population. This kit is optimized for the isolation of NCAM1/CD56-positive cells from normal human peripheral blood mononuclear cells (PBMCs). Your PBMCs are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. The NCAM1/CD56 positive cells are immobilized along the side of the tube by the magnet, while undesired NCAM1/CD56 negative cells remain in suspension for easy removal.
- Peripheral blood mononuclear cells (PBMCs) (BPS Bioscience #79059)
- Cell Isolation Magnetic Tube Rack (BPS Bioscience #78571)
- Centrifuge
- 15 or 50 ml tubes
- Cell counter
1 x 108 Cells
| Catalog # | Name | Amount | Storage |
| Cell Isolation Magnetic Beads | 200 µl | +4°C | |
| NCAM1/CD56 Cell Isolation Antibody | 40 µl | -20°C | |
| 78563 | 5x Cell Isolation Buffer | 25 ml | +4°C |
1 x 109 Cells
| Catalog # | Name | Amount | Storage |
| Cell Isolation Magnetic Beads | 2000 µl | +4°C | |
| NCAM1/CD56 Cell Isolation Antibody | 400 µl | -20°C | |
| 78563 | 5x Cell Isolation Buffer | 250 ml | +4°C |
Natural Killer (NK) cells are effector cells of the innate immune system. These lymphocytes display cytotoxic activity when activated and are of interest in the field of cancer immunotherapy. NCAM1/CD56 is a canonical marker of NK cells and is present in most stages of NK cell development. In PBMCs derived from healthy individuals, about 10-30% of the cells are NCAM1/CD56+ NK cells.
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