MYLK3, GST-tag Recombinant

Catalog #

40136

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human MYLK3, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

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Synonyms

caMLCK; MLCK, MLCK2, MGC126319, MGC126320

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

135 kDa

Amino Acids

full length

Genbank #

BC109097

UniProt #

Q32MK0

Tag(s)

N-terminal GST-tag

Background

MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure (1). Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly (2). Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium- and calmodulin-dependent.

References

1. Chan, J. Y. et al:Identification of cardiac-specific myosin light chain kinase. Circ. Res.2008; 102: 571-580.
2. Seguchi, O. et al: A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. J. Clin. Invest. 2007;117: 2812-2824.

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MYLK3, GST-tag Recombinant

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