Acetyl-Coenzyme A Carboxylase 1 (ACC1) Assay Kit

Catalog #

79315

$900 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Acetyl-Coenzyme A Carboxylase (ACC1) Assay Kit is designed to measure ACC1 activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ACC1, ATP, acetyl-CoA, sodium bicarbonate and assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against Acetyl-Coenzyme A Carboxylase 1 (ACC1)? Check out our Metabolic Enzyme Screening Services.

This product has been cited 4 times.

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Synonyms

acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1, acetyl-coa carboxylase, acetyl coa , acetyl coenzyme a

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Product Data Gallery

Inhibition of ACC1 activity by PF-05175157

Product Info

Storage and Usage

Citations4

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips

Format

Catalog #	Name	Amount	Storage
50202	ACC1, FLAG-His-Tags*	10 µg	-80°C
79283	5x ACC Assay Buffer	1 ml	-20°C
79686	500 µM ATP	100 µl	-20°C
	2 mM Acetyl-CoA	25 µl	-20°C
	1M Sodium Bicarbonate	75 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q13085

Background

ACC1 (acetyl-coenzyme A carboxylase 1) is one of two isoforms of acetyl-CoA carboxylase. It is cytosolic and it is involved in ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in de novo fatty acid synthesis, and it is found predominantly in the liver and adipose tissue. Its function is regulated by phosphorylation, allosteric regulators and other proteins, in response to the energetic needs of cells. Acetyl-CoA is at the crossroads between multiple metabolic pathways, so ACC1 has an impact in the formation of building blocks for new cells and in the response to metabolic stress. ACC1 has been linked to several diseases, such as cancer, diabetes, NAFLD (non-alcholic fatty liver disease) and obesity. Inhibition of ACC1 by TOFA (5-tetradecyloxy-2-furoic acid) can result in complete blockage of DNL (de novo lipogenesis) and may be a potential therapy for patients with NAFLD. The development of inhibitors specific for ACC1, for instance by targeting their catalytic domains or dimerization, may prove beneficial in the treatment of ACC1-related diseases.

References

Tong L. J., 2006 Cell. Biochem. 99(6):1476-1488.
Li S., et al., 2022 Nature Communications 13: 3998.
Wang Y., et al., 2022 Front Oncol. 12: 836058.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Metabolic Enzymes

Regulatory Status

Research Use Only