MAPK14 (p38α), His-Tag, Active Recombinant

Catalog #

40243

$385 *

Size: 20 µg

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Description

Mitogen-activated protein kinase 14 (MAPK14) GenBank Accession No. NM_001315, a.a. 2-360 (end), with N-terminal His-tag, MW 42.3 kDa, expressed in a baculovirus-infected Sf9 cell expression system. This protein is activated by in vitro phosphorylation and SEC.

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Synonyms

Mitogen-activated protein kinase 14, p38-alpha, p38α, p38

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

MAPK14 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

MAPK14 (His-2-360(end))

Host Species/Expression System

Sf9

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT

42 kDa

Amino Acids

2-360 (end)

Specific Activity

≥117 pmol/min/μg

Genbank #

NM_001315

UniProt #

Q16539

Tag(s)

N-terminal His-tag

References

1. Hatherley, D., et al., Mol. Cell, 2008, 31:266-77
2. Baba, N., et al., J. Exp. Med., 2013, 210(6):1251-63

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase Buffer 1 (BPS Bioscience #79334), 40 µM ATP, and 0.1 mg/ml P38 peptide substrate (InnoPep). Reaction was incubated at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40243 Lot 220901

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MAPK14 (p38α), His-Tag, Active Recombinant

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