LSD1 (KDM1A), Variant 2, GST-tag Recombinant

Catalog #

50100

$475 *

Size: 50 µg

Qty

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Description

Recombinant human LSD1 (lysine demethylase 1A) encompassing amino acids 158-852 (end). In this construct the N-terminal end of the protein is fused with GST. A short peptide linker placed between GST and the protein includes a PreScission Protease Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

This product has been cited 13 times.

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Synonyms

KDM1A, AOF2, lysine (K)-specific demethylase 1A, amine oxidase (flavin containing) domain 2, LSD, Flavin-containing amine oxidase domain-containing protein 2, AOF2, CPRF, KDM1, LSD1, BRAF35-HDAC complex protein BHC110, BHC110, [histone H3]-dimethyl-L-lysi

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

LSD1 Activity

LSD1 (KDM1A) inhibition curve using an AlphaLISA assay (#50108). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations13

Species

Human

Construct

LSD1 (GST-Pre-158-852(end))

Host Species/Expression System

E. coli

Purity

≥78%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol

104 kDa

Amino Acids

158-852(end)

Genbank #

NM_015013

UniProt #

O60341

Tag(s)

N-terminal GST-tag

References

1. Lan,F. et al., Nature 448, 7154, 718-722, 2007.
2. Forneris,F. et al., J. Biol. Chem. 282, 28, 20070-20074, 2007.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to the LSD1 (KDM1A) Chemiluminescent Assay Kit (BPS Bioscience #50109) with various amounts of LSD1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only