HER2 Kinase Assay Kit

Catalog #

40721

$750 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

HER2 (human epidermal growth factor receptor 2) encoded by ERBB2 gene has been found to be overexpressed in certain aggressive breast cancers, suggesting it as an important drug target as well as a biomarker. The HER2 Kinase Assay Kit is designed to measure HER2 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

This product has been cited 7 times.

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Synonyms

HER2, NEU, ErbB2, TKR1, CD340, human epidermal growth factor receptor 2, HER-2

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Product Data Gallery

Product Info

Storage and Usage

Citations7

Assay Kit Format

Luminescent

Supplied As

The HER2 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant HER2 enzyme, HER2 substrate, ATP and Kinase Buffer 1 for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega, #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40230	HER2	15 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	PTK substrate Poly(Glu: Tyr 4: 1) (10mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.