Rat ITK, GST-tag Recombinant

Catalog #

40447

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Rat recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_001108825), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

ITK, IL2-inducible T-cell kinase , Lyk, TSK, ITK

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

ITK, rat

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

Sf9 insect cells

Purity

≥58%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.

57 kDa

Amino Acids

352–620(end)

Specific Activity

1.0 pmol/min/μg

Genbank #

NM_001108825

UniProt #

D4A7W7

Tag(s)

N-terminal GST-tag

References

1. Hawkins, J. and Marcy, A. (2001). Protein Expr. Purif. 22 (2): 211–9.
2. Kosaka,Y., et al. (2006). Trends Immunol. 27 (10): 453–60.
3. Tanaka, N., et al. (1993). FEBS Lett. 324 (1): 1–5.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Z-Lyte^® (Invitrogen) screening protocol and condition were used. Enzyme reaction was conducted at 30°C for 1 hours with 2 μM Tyr 01 Peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl₂, 1 mM EGTA. After the 1 hour incubation, diluted Development Reagent B was added.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40447_Lot100623.pdf

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Rat ITK, GST-tag Recombinant

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