CD70-CHO Recombinant Cell line

Catalog #: 79510
Size: 2 vials
$5,500
Background:
CD70, also known as TNFSF7, is a cytokine which binds to CD27. CD70 is involved in T-cell activation and induces the proliferation of costimulated T-cells. Moreover, TNFSF7 enhances the generation of cytolytic T-cells. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. Diseases associated with CD70 include acute myocarditis, arthritis, and other inflammatory disorders.
Description:

Recombinant CHO-K1 cells constitutively expressing human CD70 (also known as Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, and CD27LG; GenBank accession #NM_001252).

Synonym(s):
Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, CD27LG
Supplied As:
Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO
Mycoplasma Testing:
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza #LT07-118) to confirm the absence of Mycoplasma contamination.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml G418 (Thermo Fisher, #11811031). 

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt
Application(s):
This cell line is useful for CD70 binding assays, flow cytometry, or for screening for CD70 antibodies. Also useful for immune checkpoint co-stimulatory assay when co-cultured with CD27/NF-κB Reporter-Jurkat cell line (BPS Bioscience #79509).
Notes:

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Immunotherapy
Product Type:
Cell line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79510.pdf