CHO-K1 cell line expressing full length human CD27 (TNFRSF7). Expression is confirmed by real time qPCR, Western blot, and flow cytometry.
Screening for antibodies recognizing CD27 and screening antibodies for binding affinities. Since CHO-K1 cells do not express endogenous CD27, this cell line serves as an excellent model for studying changes in CD27-mediated signaling pathways, such as NF-kB, MAPK and SAPK/JNK.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Each vial contains ~2 X 106 cells in 1 ml of 10% DMSO.
Format
Aqueous solution containing DMSO
UniProt #
P26842
Mycoplasma Testing
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat.
#LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set
(Lonza, Cat. #LT07-518) was used as a positive control.
Background
CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the
T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 is
expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-
kB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed
by numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27
signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and the
induction of long-term memory. The CD27/CD70 pathway is a key target for the development of
treatments for cancer and inflammatory diseases.
References
1. Akiba H et.al. (1998) CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-kB and stress-activated protein kinase/ c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-kB inducing kinase. J. Biol. Chem. 273: 13353
2. Yamamoto H et.al. (1998) NF-kB activation in CD27 signaling: Involvement of TNF receptor-associated factor in its signaling and identification of functional region of CD27. J. Immunology 161: 4753-4759
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
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