GITRL:GITR[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72061
Size: 96 reactions
$1,040
Background:
Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno oncology therapies.
Description:
The GITR:GITRL [Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of GITR:GITRL signaling. This kit comes in a convenient 96 well format, with biotin-labeled GITR (CD357), purified GITRL, streptavidin-labeled HRP, and Immunobuffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled GITR by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, GITRL is coated on a 96-well plate. Next, GITR is incubated with GITRL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Synonym(s):
Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, activity assay kit, Strep HRP, streptavidin
Format:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
One year from date of receipt when stored as directed.
Warning(s):
Avoid freeze/thaw cycles
Product Type:
Assay Kit
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Product Datasheets

72061.pdf