Human FLT3 Ligand Recombinant

Catalog #

90137-A

$130 *

Size: 2 µg

Qty

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Description

Recombinant FLT-3 Ligand is a disulfide-linked monomeric protein consisting of 156 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human FLT-3 ligand mature chain was expressed in E. coli.

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Synonyms

Fms-Related Tyrosine Kinase 3 Ligand, FLT3L, CD135L

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.

17 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

27–181

Biological Activity

The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of Human AML5 cells to be in the range of 1.0 ng/ml.

Genbank #

P49771

UniProt #

P49771

Background

FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3.The human FLT-3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. In combination with SCF and IL-3, FLT-3 ligand can cause expansion of cells with the marker spectrum CD34(+) CD38(-). Alone, FLT-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.

References

1. Cancer Res. 2009 Oct 1,69(19):7747-55.
2. Blood, Jul 2009, 114: 835 - 843.
3. J. Immunol., Jun 2009, 182: 7408 - 7414.

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Human FLT3 Ligand Recombinant

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