Flt3, His-tag Recombinant

Catalog #

40225

$375 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human FLT3 (fms related receptor tyrosine kinase 3), encompassing amino acids 564-993 (end). The construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

Flt3, FMS-like tyrosine kinase 3, CD135, FLK2, Stem Cell Tyrosine Kinase 1, STK1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

FLT3 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

Flt3 (His-564-993(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, variable imidazole, 50% glycerol, and 3 mM DTT

55 kDa

Amino Acids

564–end

Genbank #

NM_004119

UniProt #

P36888

Tag(s)

N-terminal His-tag

References

1. Takahashi,S., et al., Leuk. Res. 29 (8), 893-899 (2005).
2. Levis,M., et al., Blood 106 (2), 673-680 (2005).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to FLT3 Kinase Assay Kit (BPS Bioscience #79797) with 20 µM ATP and FLT3 titrated from 75 ng/rxn to 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40225 Lot 220121-1

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