FLT3 Kinase Assay Kit

Catalog #

79797

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FLT3 Kinase Assay Kit is designed to measure FLT3 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

Need us to run inhibitor screens or profile your compounds against FLT3? Check out our Kinase Screening Services.

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Synonyms

Fms-Related Tyrosine Kinase 3 Ligand, FLT3L, CD135L

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The FLT3 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant FLT3 enzyme, substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Reagent	Amount	Storage
40225	FLT3	2 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
	Myelin basic protein (MBP) (5 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P36888

References

1. Swords, R., Freeman, C., and Giles, F. 2012. "Targeting the FMS-like tyrosine kinase 3 in acute myeloid leukemia." Leukemia 26(10): 2176-2185.
2. Parcells, B.W., et al. 2006. "FMS‐like tyrosine kinase 3 in normal hematopoiesis and acute myeloid leukemia." Stem cells 24(5): 1174-1184.

Storage/Stability

Up to 6 months when stored as recommended.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only