The VEGFR3 Kinase Assay Kit is designed to measure VEGFR3 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
The VEGFR3 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant VEGFR3 enzyme, VEGFR3 substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
ADP-Glo® Kinase Assay (Promega #V6930) Dithiothreitol (DTT, 1 M; optional) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips 30°C incubator
Format
UniProt #
P35916
Background
Vascular endothelial growth factor receptor 3 (VEGFR3), also called Fms-Related Tyrosine Kinase 4 (FLT4), is a tyrosine kinase (TK) receptor for VEGFs that plays a central role in tumor angiogenesis; therefore, the inhibition of VEGFR3 is a promising therapeutic strategy for inhibiting angiogenesis and tumor growth.
References
Yeh, Yu-Wen et al. “Targeting the VEGF-C/VEGFR3 axis suppresses Slug-mediated cancer metastasis and stemness via inhibition of KRAS/YAP1 signaling” Oncotarget vol. 8,3 (2016): 5603-5618.
Silvia M. Ferrari, et al. “Lenvatinib in the Therapy of Aggressive Thyroid Cancer: State of the Art and New Perspectives with Patents Recently Applied”, Recent Patents on Anti- Cancer Drug Discovery (2018) 13: 201.
Storage/Stability
Up to 6 months when stored as recommended.
Instructions for Use
See assay protocol for detailed instructions
Shipping Temperature
-80°C
Warnings
Avoid multiple freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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