FES, His-FLAG-tag Recombinant

Catalog #

40425

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human feline sarcoma oncogene (FES), GenBank Accession # NM_002005, a.a. 2-822 (end), N-terminal His-tag and N-terminal FLAG-tag, MW=95.3 kDa, expressed in Sf9 insect cells via a baculovirus expression system

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Synonyms

feline sarcoma oncogene, FES

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

FES

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 3 mM DTT, 2.2 mM KCl, 20% glycerol, 80 µg/ml FLAG peptide.

125 kDa

Amino Acids

full length

Specific Activity

235 pmol/min/µg

Genbank #

NM_002005

UniProt #

P07332

Tag(s)

N-terminal His-FLAG-tags

References

1. Hellwig S, Smithgall TE. Front Biosci. 2011 Jun 1,17:3146-55.
2. Kanda S, Miyata Y. Front Biosci. 2011 Jan 1,16:1024-35.

Storage/Stability

>6 months at –80°C

Assay Conditions

Assay was performed in a Kinase buffer (40 mM Tris pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA) containing 1 mM DTT using Poly-(Glu4:Tyr)substrate (0.2 mg/ml) and 20 µM ATP for 30 minutes at 30°C. The amount of ATP transferred was alculated using Kinase-Glo Plus™ reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40425_Lot110630-HE.pdf 40425_Lot110630-HEFE.pdf

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FES, His-FLAG-tag Recombinant

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