ERN1 (IRE1), GST-tag Recombinant

Catalog #

40306

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human endoplasmic reticulumto- nucleus signaling 1 (ERN1), also known as inositol-requiring protein 1 (IRE1), GenBank Accession No. NM_001433, a.a. 468-977 (end) MW = 87 kDa, expressed in a baculovirus-infected Sf9 insect cell expression system.

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Synonyms

inositol-requiring protein 1 (IRE1), IRE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Baculovirus-infected Sf9 insect

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

87 kDa

Amino Acids

468-977 (end)

Specific Activity

55 pmol/min/μg

Genbank #

No. NM_001433

UniProt #

O75460

Tag(s)

N-terminal GST-tag

References

1. Niwa, M., et al., Cell. 1999; 99: 691-702.

2. Iwawaki, T. et al., Proc. Nat. Acad. Sci. 2009; 106: 16657-16662.

Storage/Stability

>6 months at -80°C

Assay Conditions

10 μl of varying amounts of ERN1 was mixed with 5 μl of 1 mg/ml myelin basic protein substrate and 5 μl of H2O. Reaction was initiated by adding 5 μl of 250 μM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying enzyme kinetics and screening small molecule inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Kinase/Serine Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40306_Lot141021.pdf

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ERN1 (IRE1), GST-tag Recombinant

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