Human IRE1, His-Avi-tag Recombinant

Catalog #

40709

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human IRE1 (protein kinase inositol-requiring enzyme 1), also known as serine/threonine-protein kinase/endoribonuclease 1, encompassing amino acids 649-977. This construct contains an N-terminal His-tag (10xHis) followed by an Avi-Tag™. The His-tag and the Avi-Tag™ are separated by an optimal TEV (tobacco etch virus) protease target sequence that allows cleavage of the His-tag and release of the Avi-IRE1 recombinant (TEV: tobacco etch virus cysteine protease #50308). The protein was affinity purified.

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Synonyms

Serine/threonine-protein kinase/endoribonuclease IRE1; ERN1; Endoplasmic reticulum-to-nucleus signaling 1; IRE1; IRE1P; IRE1a; hIRE1p

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

IRE1 (His-Avi-(469-977))

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 300 mM Imidazole

62 kDa

Amino Acids

469-977

Genbank #

NM_001433.3

UniProt #

O75460

Tag(s)

N-terminal His-Avi-tag

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was done in a Kinase buffer using MBP (NEB, 0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40709_Lot_151028.pdf

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Human IRE1, His-Avi-tag Recombinant

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