EPHB2, His-tag Recombinant

Catalog #

40200

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human EPHB2 (GenBank Accession No. NM_004442), a.a. 569-end with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

EphB2, Ephrin Type-B Receptor 2, EPH-Like Kinase 5, ELK5, HEK5, Tyro5, EPHB-2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.

52 kDa

Amino Acids

569–end

Specific Activity

21.8 pmol/min/μg.

Genbank #

NM_004442

UniProt #

P29323

Tag(s)

N-terminal His-tag

References

1. Macrae,M., et al., Cancer Cell 8 (2), 111-118 (2005).
2. Xu,H., et al., Am. J. Physiol. Renal Physiol. 288 (4), F855-F866 (2005).

Storage/Stability

>12 months at –80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (Invitrogen Zlyte Peptide 1) at room temperature for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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EPHB2, His-tag Recombinant

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