EPHB4, His-tag Recombinant

Catalog #

40201

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human EPHB4 (GenBank Accession No. NM_004444), a.a. 563-987 with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

EphB4, Ephrin Type-B Receptor 4, Hepatoma Transmembrane Kinase, HTK, Tyro11, MYK1, EPHB-4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥78%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

50 kDa

Amino Acids

563–987

Specific Activity

43 pmole/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30°C.

Genbank #

NM_004444

UniProt #

P54760

Tag(s)

N-terminal His-tag

References

1. Bruhl,T., et al., Circ. Res. 94 (6), 743-751 (2004).
2. Fuller,T., et al., J. Cell. Sci. 116 (Pt 12), 2461-2470 (2003).

Storage/Stability

>12 months at –80°C.

Assay Conditions

ADP-Glo assay with 0.2 mg/ml poly-(Glu4:Tyr) as a substrate and 100 µM ATP at final concentration. Reaction was done for 50 minutes at 30°C. ADP-Glo for 45 minutes at RT, and Kinase detection for 55 minutes at RT.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40201_Lot120719.pdf 40201_Lot120716.pdf

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