c-RAF(Y340D, Y341D), GST-Tag Recombinant

Catalog #

40008

$375 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human c-RAF (Raf-1 proto-oncogene, serine/threonine kinase), encompassing amino acids 306-648(end). This construct contains an N-terminal GST-tag. This protein contains mutations of interest (Y340D and Y341D). The recombinant protein was affinity purified.

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Synonyms

cRAF, RAF1, NS5, RAF-1, RAF

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥72%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT.

65 kDa

Amino Acids

306-648(end)

Specific Activity

≥158 pmol/min/μg

Genbank #

NM_002880

UniProt #

P04049

Tag(s)

N-terminal GST-tag

References

1. Lamberti,A. et al. Cell Death Differ. 14(5): 952-962 (2007).
2. Yu,F. et al. PLoS Pathog. 3(3): E44 (2007).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done by using cRAF Kinase Assay Kit (BPS Bioscience #79570) with RAF substrate and 10 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40008 Lot 201030-G2

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c-RAF(Y340D, Y341D), GST-Tag Recombinant

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