c-Mer, GST-tag (Human) Recombinant

Catalog #

40254

$385 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human c-Mer, (also known as tyrosine-protein kinase Mer and MERTK) encompassing amino acids 578-872 and containing the kinase domain. This construct contains an N-terminal GST-tag. This recombinant protein was affinity purified.

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Synonyms

tyrosine-protein kinase Mer, MERTK, receptor tyrosine kinase MerTK

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

c-Mer (GST-578-872)

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione

60 kDa

Amino Acids

578–872

Specific Activity

≥50 pmol/min/µg

Genbank #

NM_006343

UniProt #

Q12866

Tag(s)

N-terminal GST-tag

Background

c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family (1). The c-MER gene encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain. Functional knockout of c-MER in mice shows the mice to have macrophages deficient in the clearance of apoptotic thymocytes thereby demonstrating the critical role for c-MER in the engulfment and efficient clearance of apoptotic cells. Defects in c-MER have been associated with retinitis pigmentosa, a phenotype that can be reversed by replacement of the c-MER gene product (2).

References

1. Van Hoof, C. et al., Genomics. 1995; 28: 261-272.
2. Chao, Y. et al., Molec. Cell. 2006; 23: 535-546.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in kinase buffer containing 1 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and for inhibitor screening.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only