BTK (C481S) Kinase Assay Kit
The BTK (C481S) Kinase Assay Kit is designed to measure BTK (C481S) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
- ADP-Glo® Kinase Assay (Promega #V6930)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 101039 | BTK (C481S), His-Tag* | 10 µg | -80°C |
| 79334 | 5X Kinase Assay Buffer 1 | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 100 µl | -20°C |
| 40217 | PTK substrate Poly (Glu:Tyr: 4:1) (10 mg/ml) | 100 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
Bruton’s tyrosine kinase (BTK), is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. However, as therapies have progressed, specifically in chronic lymphocytic leukemia (CLL), resistance has become more common in patients where the disease has progressed due to prolonged therapy. A mutation in the BTK 481 cysteine residue to which inhibitors bind covalently is often linked to BTK inhibition (BTKi) in CLL patients where resistance has been led to disease progression. Targeting the BTK mutation of interest (C481S) has shown promising clinical efficacy in treating CLL patients that are no longer responding to common therapy.
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