Anti-CD4 Antibody, Biotin-Labeled

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Catalog #

101761

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Description

This purified recombinant antibody is a humanized mouse IgG4κ anti-CD4 (cluster of differentiation 4) antibody that selectively binds to CD4. Its sequence is similar to Ibalizumab. This antibody has a C-terminal Avi-Tag™ on its heavy chain. This antibody has been tested for specific binding to human CD4 in an in vitro ELISA.

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Synonyms

CD4, Cd4, T-cell surface antigen T4/Leu-3, T-cell differentiation antigen L3T4, T-cell surface glycoprotein CD4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD4: Anti-CD4-Biotin Binding Assay

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Mouse

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 51 kDa; Light Chain: 24 kDa + glycans

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

P06332

Label

This antibody is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

CD4 (cluster of differentiation 4) is part of the immunoglobulin superfamily, and it can be found in T-helper cells, monocytes, macrophages and dendritic cells. It is a co-receptor in the TCR (T-cell receptor), binding to MHC (major histocompatibility complex) class II molecules. CD4 binds to the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase), which can phosphorylate the ITAM (immunoreceptor tyrosine activation motifs) domain of the CD3, activating CD3 related signaling. CD4 is a typical T cell marker of T helper cells, and it has been linked to cancer, autoimmune diseases such as vitiligo and type I diabetes. In addition, HIV-1 makes use of CD4 to trigger viral envelope protein conformational changes that allow cell infection. Ibalizumab, an anti-CD4 antibody, is currently used in the treatment of HIV, being considered a first-in-class medication. Further studies into CD4 and potential strategies around it may benefit patients and CD4 related diseases.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The antibody was validated by measuring anti-CD4 binding to human CD4 antigen in ELISA. The CD4 protein (BPS Bioscience #101862) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD4 antibody (BPS Bioscience #101761), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. The plate was washed and incubated with Streptavidin-HRP, washed again, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying the binding to CD4 in ELISA

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immune Cell Signaling

Regulatory Status

Research Use Only