AMPK (A1/B1/G1), His-tags

Catalog #: 40025
Size: 10 µg
$595
Species: Human
Host Species: Sf9 insect cells
MW: ~146 kDa (complex)
Genbank #: NM_006251(A1), NM_006253(B1), NM_002733 (G1)
Tag(s): C-terminal His-tags
a.a: full length
Background:
AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
Description:
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. The gene accession number for the three subunits (A1/B1/G1) is NM_006251, NM_006253, and NM_002733, respectively. This protein has been activated with CAMKK.
Synonym(s):
Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G1: PRKAG1, AMPKG, MGC8666
Purity:
≥95%
Specific Activity:
≥700 pmole/min/µg
Assay Conditions:
Assay was done in Kinase buffer containing 1 mM DTT using AMARA peptide as a substrate (0.1 mg/ml), 20 μM ATP and 100 μM AMP . Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase- Glo reagent (Promega)
Formulation:
50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Minokoshi, Y. et al. Nature 428: 569-574, 2004.
2. Hardie, D G. et al. Eur J Biochem. 1997 Jun 1,246(2):259-73.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
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Product Datasheets

40025_Lot 150427.pdf