Abl Kinase, His-tag

Catalog #: 40411
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 33 kDa
Genbank #: NM_00731
Tag(s): N-terminal His-tag
a.a: 248–531
Description:
Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 33 kDa, expressed in a Baculovirus-infected Sf9 cell expression system
UniProt
P00519
Synonym(s):
ABL1, ABL-1, Abelson murine leukemia viral oncogene homolog 1, JTK7
Specific Activity:
2,700 pmoles/min/μg.
Assay Conditions:
Assay was performed in Kinase buffer containing 0.2 mM DTT using Abltide substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM imidazole, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356–364.

Application Reference:
LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia (2014)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Receptor Tyrosine
Product Type:
Recombinant Protein
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