TIGIT (Mouse): CD155 (Mouse) Homogeneous Assay Kit

Catalog #

78141

$495 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Mouse TIGIT: Mouse CD155 (mTIGIT:mCD155) Homogeneous Assay Kit is designed to measure the inhibition of mTIGIT binding to mCD155. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing mTIGIT and an inhibitor of choice is incubated with the mCD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

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Synonyms

T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3, PVR, NECL-5, CD155-His

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Species

Mouse

Supplied As

The mTIGIT:mCD155 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated Mouse TIGIT, His-tagged Mouse CD155, and assay buffer to perform a total of 384 reactions.

Materials Required But Not Supplied

Name	Catalog #
AlphaLISA Ni Chelate Acceptor beads, 5 mg/ml	PerkinElmer #AL108C
AlphaScreen Streptavidin-conjugated Donor beads, 5 mg/ml	PerkinElmer #6760002S
Optiplate-384	PerkinElmer #6007290
AlphaScreen microplate reader
Adjustable micropipettor and sterile tips

Format

Catalog #	Name	Amount	Storage
79269	Mouse TIGIT-Fc-biotin	2x 3 µg	-80°C
71167	Mouse CD155-His	2x 5 µg	-80°C
79311	3x Immuno Buffer 1	4 ml	-20°C

Background

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor that is expressed on the surface of T cells and NK cells that binds to CD155 and CD112 on the surface of dendritic cells. Binding of TIGIT with CD155 or CD112 results in inhibition of T cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have been shown to increase the immune response, especially in the case of certain cancers.

References

1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.
2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. 2009; 106(42): 17858-17863.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Instructions for Use

See assay protocol for detailed instructions

Shipping Temperature

-80°C

Notes

AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.

Contraindications

Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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TIGIT (Mouse): CD155 (Mouse) Homogeneous Assay Kit

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