CD155 / TCR Activator - CHO Recombinant Cell line
Catalog #
60548
$10,855
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Description
Recombinant CHO-K1 cells constitutively expressing human CD155 (Poliovirus receptor, Nectin-like protein 5, GenBank accession # NM_006505) and an engineered T cell receptor (TCR) activator.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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Synonyms
Poliovirus receptor, Nectin-like protein 5, CD155-His
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Product Data Gallery
Anti-TIGIT neutralizing antibody induced NFAT luciferase reporter activity in TIGIT/NFAT Reporter-Jurkat cells, but not in control NFAT Reporter-Jurkat cells, when co-cultured with CD155/TCR Activator-CHO cells
Anti-TIGIT neutralizing antibody induced NFAT luciferase reporter activity in TIGIT/NFAT Reporter-Jurkat cells when co-cultured with CD155/TCR Activator-CHO cells, but not with control TCR Activator-CHO cells
FACS analysis of cell surface expression of CD155 in CD155/TCR Activator-CHO cells
Product Info
Storage and Usage
Citations
Host Cell Line
CHO
Materials Required But Not Supplied
Materials Required for Cell Culture
- Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) supplemented with 10% FBS, 1% Penicillin/Streptomycin
- Growth Medium 3C (BPS Bioscience #79537)
Materials Required for Cellular Assay
- TIGIT/NFAT Reporter - Jurkat Cell Line (BPS Bioscience #60538)
- Assay Medium 3A (BPS Bioscience #79816)
- Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) supplemented with 10% FBS, 1% Penicillin/Streptomycin
- Anti-TIGIT neutralizing antibody (BPS Bioscience #71340)
- 96-well tissue culture-treated white clear-bottom assay plate
- One-Step luciferase assay system (BPS Bioscience #60690) or other luciferase reagents for measuring firefly luciferase activity
- Luminometer
UniProt #
P15151
Mycoplasma Testing
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Background
TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.
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