PI3Kβ (p110β/p85α) Assay Kit

Catalog #

79802

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PI3Kβ (p110β/p85α) Assay Kit is designed to measure PI3Kβ activity for screening and profiling applications, using ADP-Glo® Kinase Assay as a detection reagent.

Need us to run inhibitor screens or profile your compounds against PI3Kβ (p110β/p85α)? Check out our Kinase Screening Services.

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Synonyms

PIK3CB, p110beta, Phosphoinositide-3-Kinase Catalytic Beta

PIK3R1, Phosphoinositide-3-Kinase Regulatory Subunit 1 (Alpha), GRB1, p85alpha, PI3K, pi3k

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Species

Human

Supplied As

The PI3Kβ (p110β/p85α) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PI3Kβ enzyme, PI3K lipid substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Reagent	Amount	Storage
40622	PI3Kβ (p110β/p85α)	5 µg	-80°C	Avoid mutiple freeze/ thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
40560	PI3K lipid substrate (Packaged separately, Do Not Freeze!)	500 µl	+4°C
79696	96-well plate, white	1	Room Temp.

UniProt #

p110ß: P42338; p85α: P27986

Background

PI3Ks (Phosphatidylinositol 3-kinases) are lipid kinases that phosphorylate PIP2 (phosphatidylinositol 4,5-bisphosphate) to produce PIP3 (phosphatidylinositol 3,4,5-trisphosphate), which plays important roles in fundamental cellular activities such as cell growth, survival, migration, and metabolism. In human cancers, gain-of-function mutations of PI3Ks are found frequently, suggesting that PI3Ks are closely involved in tumorigenesis and that PI3K targeting inhibitors may be promising anticancer drug candidates.

References

Zhao W., et al. Acta Pharmaceutica Sinica B, 7(1): 27-37 (2017)

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Contraindications

Avoid >0.5% DMSO. Higher DMSO levels can significantly decrease the enzyme activity.

Warnings

Avoid freeze thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only