The PI3Kδ (p110δ/p85α) Assay Kit is designed to measure PI3Kδ (PI3K-delta) activity for screening and profiling applications, using ADP-Glo® Kinase Assay as a detection reagent.
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The PI3Kδ (p110δ/p85α) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PI3Kδ enzyme, PI3K lipid substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
ADP-Glo® Kinase Assay (Promega #V6930) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips 30°C incubator
PI3Ks (Phosphatidylinositol 3-kinases) are lipid kinases that phosphorylate PIP2 (phosphatidylinositol 4,5-bisphosphate) to produce PIP3 (phosphatidylinositol 3,4,5-trisphosphate), which plays important roles in fundamental cellular activities such as cell growth, survival, migration, and metabolism. In human cancers, gain-of-function mutations of PI3Ks are found frequently, suggesting that PI3Ks are closely involved in tumorigenesis and that PI3K targeting inhibitors may be promising anticancer drug candidates.
References
Zhao W., et al. Acta Pharmaceutica Sinica B, 7(1): 27-37 (2017)
Storage/Stability
Up to 6 months when stored as recommended.
Instructions for Use
See assay protocol for detailed instructions.
Shipping Temperature
-80°C
Contraindications
Avoid >0.5% DMSO. Higher DMSO levels can significantly decrease the enzyme activity.
Warnings
Avoid multiple freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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