PI3 Kinase [p110α (E545K)/p85α], His-Tag Recombinant

Catalog #

40644

$465 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Complex of recombinant full-length human p110α (GenBank Accession No. NM_006218) with E545K mutation and N-terminal His-Tag, and recombinant full length, human p85α (GenBank Accession No. NM_181523), no tag. Co-expressed in Sf9 cells in a Baculovirus expression system. p110α (E545K) MW=111 kDa, p85α MW=86 kDa.

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Synonyms

Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha; p110alpha, Phosphoinositide 3-kinase alpha, PI3-K, pi3K, GRB1, PIK3R1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Formulation

50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

p110α (E545K): 111 kDa, p85α: 86 kDa

Amino Acids

Full-length

Genbank #

p110α (E545K): NM_006218, p85α: NM_181523

UniProt #

p110α: P42336; p85α: P27986

Tag(s)

p110α: N-terminal His-Tag, p85α: No Tag

References

1. Samuels, Y., et al. Science 304: 554 (2008)

2. Graupera, M., et al., Nature 453: 662-666 (2008)

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Datasheets/Protocols

40644_Lot161215.pdf

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PI3 Kinase [p110α (E545K)/p85α], His-Tag Recombinant

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