Yes1, GST-tag Recombinant

Catalog #

40488

$385 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human, full length, YES1, also known as Yes, c-yes, HsT441, P61-YES GenBank Accession No. NM_005433, with N-terminal GST tag, a.a. 2- 543(end), MW 88 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

This product has been cited 4 times.

●

Synonyms

Yes, c-yes, HsT441, P61-YES

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations4

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.

91 kDa

Amino Acids

full length

Specific Activity

200 pmole/min/µg

Genbank #

NM_005433

UniProt #

P07947

Tag(s)

N-terminal GST-tag

Background

YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene that has tyrosine kinase activity and belongs to the SRC family. YES1 lies in close proximity to thymidylate synthase gene on chromosome 18 and chromosome 22 (1). The activation of YES1 may play a significant role in the malignant transformation of hepatocytes, and is important for maintaining embryonic stem cells in an undifferentiated state. YES1 is a useful marker to detect early-stage hepatocellular carcinoma, and it plays a key role in the tumorigenesis and metastasis of gastric cancer. YES1 induction results in increased cancer cell motility suggesting that YES1 may promote cancer spread and metastasis rather than tumor growth (2).

References

1. Silverman, G. et.al, Genomics 15: 442-445, 1993.
2. Barraclough, J. et al, Neoplasia. 2007 Sep;9(9):745-54.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40488_Lot140319-1R.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

Yes1, GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY