Yes1, GST-tag

Catalog #: 40488
Size: 10 µg
$375
Species: Human
Host Species: Sf9 insect cells
MW: 91 kDa
Genbank #: NM_005433
Tag(s): N-terminal GST-tag
a.a: full length
Background:
YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene that has tyrosine kinase activity and belongs to the SRC family. YES1 lies in close proximity to thymidylate synthase gene on chromosome 18 and chromosome 22 (1). The activation of YES1 may play a significant role in the malignant transformation of hepatocytes, and is important for maintaining embryonic stem cells in an undifferentiated state. YES1 is a useful marker to detect early-stage hepatocellular carcinoma, and it plays a key role in the tumorigenesis and metastasis of gastric cancer. YES1 induction results in increased cancer cell motility suggesting that YES1 may promote cancer spread and metastasis rather than tumor growth (2).
Description:
Human, full length, YES1, also known as Yes, c-yes, HsT441, P61-YES GenBank Accession No. NM_005433, with N-terminal GST tag, a.a. 2- 543(end), MW 88 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

UniProt
P07947
Synonym(s):
Yes, c-yes, HsT441, P61-YES
Specific Activity:
200 pmole/min/µg
Assay Conditions:
Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Silverman, G. et.al, Genomics 15: 442-445, 1993.
2. Barraclough, J. et al, Neoplasia. 2007 Sep;9(9):745-54.

Application Reference(s):
1. An iterative compound screening contest method for identifying target protein inhibitors using the tyrosine-protein kinase Yes (2017)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Cytoplasmic Tyr
Product Type:
Recombinant Protein
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Product Datasheets

40488_Lot140319-1R.pdf