YES1 Assay Kit
Proto-oncogene tyrosine-protein kinase YES1 has been implicated in regulation of cell growth and survival, apoptosis, cell-cell adhesion, and differentiation. It has been identified as a potential target in basal-like breast cancers. The YES1 Assay Kit is designed to measure YES1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The YES1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant YES1 enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number | Component | Amount | Storage | |
| 40488 | YES1, GST-tag | 10 µg | -80°C | Avoid freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
| 79686 | ATP (500 µM) | 100 µl | -20°C | |
| 40217 | Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) | 100 µl | -20°C | |
| 79696 | 96-well plate, white | 1 | Room Temp. | |
1. Hamamura K., Tsuji M., et al. Functional Activation of Src Family Kinase Yes Protein is Essential for the Enhanced Malignant Properties of Human Melanoma Cells Expressing Ganglioside GD3; 2011, J.] Biol. Chem.] 286: 18526-18537.
2. Bilal, E., Alexe G., et al. Identification of the YES1 Kinase as a Therapeutic Target in Basal-Like Breast Cancers; 2010, Genes & Cancer Oct; 1(10): 1063–1073.
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