USP51, SUMO-His-tags Recombinant

Catalog #

81103

$425 *

Size: 25 µg

Qty

Bulk/Custom

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Description

Human USP51 recombinant protein (Genbank Accession No. Q70EK9), with N-terminal SUMO- and His-tags, expressed in E. coli.

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Synonyms

Deubiquitinating enzyme 51, Ubiquitin carboxyl-terminal hydrolase 51, Ubiquitin-specific-processing protease 51, Ubiquitin thioesterase 51, USP 51, USP-51

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM DTT, 10% glycerol

56 kDa

Genbank #

Q70EK9

UniProt #

Q70EK9

Tag(s)

N-terminal SUMO-His-tags

Background

USP51, a member of the USP family of cysteine proteases, contains a UBP-type Zn-finger and is expressed in the prostate, brain lung, aorta, and kidney.

References

Quesada, V., et al., Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases. Biochem Biophys Res Commun. 314:54-62 (2004)

Storage/Stability

Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.

Assay Conditions

Assay Conditions: 50 nM USP51, 200 nM substrate, 50 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM DTT, 0.05% CHAPS

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

This enzyme is active in the Ub-CHOP assay.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deubiquitinase

Regulatory Status

Research Use Only

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USP51, SUMO-His-tags Recombinant

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