USP10, FLAG-tag Recombinant

Catalog #

80360

$425 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human USP10 (Ubiquitin-Specific Protease 10) or Ubiquitin C-terminal Hydrolase 10, GenBank Accession No. NM_005153, a.a. 2-798(end) with an N-terminal FLAG-tag, MW=88 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

This product has been cited 1 time.

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Synonyms

USP 10, Ubiquitin-Specific Protease 10, Ubiquitin C-terminal Hydrolase 10, Ubch10, USP-10

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

USP10 inhibition curve using a fluorogenic assay (#79094). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

88 kDa

Amino Acids

2–798(end)

Specific Activity

1.5 pmole/min/µg

Genbank #

NM_005153

UniProt #

Q14694

Tag(s)

N-terminal FLAG-tag

References

1. Faus, H., et al. (2005). Mol. Cell. Endocrinol. 245(1-2):138-46.
2. Soncini, C., et al. (2001). Oncogene 20(29):3869-79.
3. Yuan, J., et al. (2010). Cell 140(3): 384-96.

Application Reference:
Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1 (2014)

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP10 in a 50 µL volume. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80360_Lot140501-2.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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USP10, FLAG-tag Recombinant

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