UCHL1 (PGP9.5), His-tag Recombinant

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Catalog #

80351

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Description

Human Ubiquitin Carboxyterminal Hydrolase (UCHL1), also known as PGP9.5, GenBank Accession No. NM_004181, a.a. 2-223, with N-terminal His-tag, expressed in E. coli. expression system. MW=26 kDa.

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Synonyms

UCHL1, Ubiquitin Carboxy-terminal Hydrolase L1, PGP9.5, Ubiquitin C-terminal Hydrolase L1, UCHL-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

UCHL1 inhibition curve using a fluorogenic assay (#78833). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 10% Glycerol and Imidazole.

26 kDa

Amino Acids

2–223(end)

Specific Activity

6000 pmol/min/μg

Genbank #

NM_004181

UniProt #

P09936

Tag(s)

N-terminal His-tag

References

1. Gong, B., et al., (2006). Cell 126 (4): 775– 788.
2. Healy DG, et al., (2005). Cell Tissue Res. 318 (1): 189–94.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL1. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin Hydrolase

Regulatory Status

Research Use Only

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Datasheets/Protocols

80351 lot 200924-1

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UCHL1 (PGP9.5), His-tag Recombinant

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