TTK, GST-tag Recombinant
Catalog #
40291
$410
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Description
Recombinant human TTK, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
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Synonyms
ESK, PYT, MPS1, MPS1L1, FLJ38280
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Product Data Gallery
4-20% SDS-PAGE Coomassie Staining
Specific Activity
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity
≥75%
Format
Aqueous buffer solution
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
MW
130 kDa
Amino Acids
full length
Genbank #
NM_003318
UniProt #
P33981
Background
TTK is a serine/threonine kinase that has been implicated in the regulation of centrosome duplication and mitotic checkpoint response. Overexpressing of dominant-negative TTK in human cell lines prevents centrosome duplication while active TTK accelerates centrosome reduplication in an osteosarcoma cell line. Disruption of TTK function leads to a combination of severe mitotic abnormalities and failure in centrosome duplication (1). TTK is required for stabilization and activation of p53 during spindle disruption. TTK phoshorylates the N-terminal domain of p53 at Thr18, and this phosphorylation disrupts the interaction with MDM2 and abrogates MDM2-mediated p53 ubiquitination (2).
References
1. Fisk H A, et al: Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression. Proc. Nat. Acad. Sci. 100: 14875-14880, 2003.
2. Huang Y F, et al: TTK/hMps1 mediates the p53-dependent postmitotic checkpoint by phosphorylating p53 at Thr18. Mol Cell Biol. 2009 Jun;29(11):2935-44.
2. Huang Y F, et al: TTK/hMps1 mediates the p53-dependent postmitotic checkpoint by phosphorylating p53 at Thr18. Mol Cell Biol. 2009 Jun;29(11):2935-44.
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