TTK (MPS-1) Kinase Assay Kit

Catalog #

78356

$470 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

TTK, also known as Monopolar Spindle 1 Kinase (MPS-1), is a dual specific kinase (tyrosine and serine/threonine) that plays an important role in the cell division cycle by regulating spindle assembly checkpoint during mitosis. Recent studies showed that inhibition of TTK induces aberrant mitosis and apoptosis in various cancer cells, suggesting that TTK is a potential cancer therapeutic target. The TTK Kinase Assay Kit is designed to measure TTK kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

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Synonyms

Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT, TTK, MPS1, MPS1L1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The TTK Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant TTK enzyme, MBP substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega, #V6930)
Dithiothreitol (DTT 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40291	TTK (MPS-1)*	10 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
78514	MBP (5 mg/ml)	100 µl	-80°C
79696	96-well plate, white	1	Room Temp

*The concentration of TTK (MPS-1) is lot-specific and will be indicated on the tube containing the enzyme

UniProt #

P33981

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Studying enzyme kinetics.
Screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only