STK4(MST1), GST-tag Recombinant

Catalog #

40017

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human STK4 (serine/threonine kinase 4), also known as MST-1 (Mammalian STE20-like protein kinase 1) or KRS2, GenBank Accession No. NM_006282, a.a. 2-488(end), with N-terminal GST-tag, MW=81.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

KRS2; YSK3; DKFZp686A2068; STK4

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥50%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol.

81.5 kDa

Amino Acids

2–488(end)

Specific Activity

73 pmole/min/µg

Genbank #

NM_006282

UniProt #

Q13043

Tag(s)

N-terminal GST-tag

Background

MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20). Endogenous full-length MST1 is activated by a variety of stressful stimuli, accompanied by the secondary appearance of a 36 kDa Thr183-phosphorylated, caspase-cleaved catalytic fragment. Recombinant MST1 undergo a robust autoactivation in vitro, mediated by an intramolecular autophosphorylation on the activation loop of an MST dimmer. MST1 can initiate apoptosis when transiently overexpressed in mammalian cells. Interference with the ability of endogenous MST1 to associate with the putative tumor suppressor proteins Nore1/RASSF can inhibit Ras-induced apoptosis.

References

1. Taylor, L.K., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(19):10099-104.
2. Song, H., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(4):1431-6.
3. Ura, S., et al. (2001). Proc. Natl. Acad. Sci. USA. 98(18):10148-53.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Ser/Thr Peptide 7, Invitrogen) at 30°C for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40017_Lot101012.pdf

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STK4(MST1), GST-tag Recombinant

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