SMARCA2, GST-tag Recombinant

Catalog #

31141

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human SMARCA2, also known as BRM, GenBank Accession No. NM_003070, a.a. 1375-1511 corresponding to single bromodomain, with N-terminal GST-tag, MW=43kDa, expressed in expressed in an E. coli expression system.

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Synonyms

SMARCA2, BRM, BRG1-Associated Factor 190B, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin 2, BAF190, SNF2L2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

SMARCA2 Activity

SMARCA2 inhibition curve using a TR-FRET assay (#40342). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

43 kDa

Amino Acids

1375–1511

Genbank #

NM_003070

UniProt #

P51531

Tag(s)

N-terminal GST-tag

References

1. Van Houdt JK., et al. Nat Genet. 2012; 44(4):445-9.
2. Wolff D., et al. Mol Syndromol. 2012; 2(6):237-244.

Storage/Stability

>6 months at –80°C.

Assay Conditions

SMARCA2 is incubated with Bromodomain Ligand 2 in SMARCA2 buffer in a 10 μl reaction for 30 minutes. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, followed by AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer). After 10 minutes incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only