Siglec4, Fc Fusion, Avi-tag, Biotin-labeled Recombinant
Human secreted Sialic Acid Binding Ig Like Lectin 4 (Siglec4)-Fc fusion protein, also known as MAG and myelin associated glycoprotein, GenBank Accession No. NM_002361, a.a. 20-516, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. MW = 83 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
≥90%
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.
For more information on enzymatic biotinylation, please see our Tech Note.
1. Sun, J., et al., Glycobiology. 2004; 14(9):851-857.
2. Lehmann, F., et al., Glycobiology. 2004; 14(11): 959-968.