Acetyltransferase Screening

Accelerate your drug discovery program with Acetyltransferase Screening and Profiling Services from BPS Bioscience, Inc. Trust us to provide you with high quality data with fast turnaround time. Our team of experts along with our broad services portfolio makes it easy to:

    • Screen for hits in large compound libraries using our acetyltransferase biochemical assays
    • Compare potency with IC50 screens
    • Receive data within days of compound submission
    • Perform follow-up studies using the same proteins manufactured in-house
    • Get questions answered or project guidance in a time-efficient manner

BPS Bioscience offers many immuno-oncology related products in addition to screening and profiling services. 

BPS Bioscience offers rapid and dependable biochemical and cell based screening of small molecules, peptides, and antibodies against requested targets by IC50 determination. Our team of experts along with our broad services portfolio make it easy to:

  • Screen for inhibitors/targets using our assays
  • Select from IC50 determination and single point concentrations
  • Receive data within days of compound submission
  • Perform follow-up studies using the same soluble receptors manufactured in-house
  • Get questions answered or project guidance in a time-efficient manner

List of Assays:

Biochemical Assay Format: 

The acetyltransferase screening assay is based on acetyltransferase-mediated transfer of an acetyl group from acetyl-CoA to a histone substrate that is coated onto a 96-well plate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader. Note: Image not drawn to scale. 

Background:

Acetyltransferases are enzymes that transfer an acetyl group from acetyl CoA to lysine amino acids. In the case of histone acetyltransferases (HATs), these lysine residues reside on histone tails. Generally, addition of acetyl groups to histone tails results in gene activation by inducing a euchromatin conformation and recruiting bromodomain-containing transcription factors for genes in close proximity to the acetylated histone. In a reaction mediated by deacetylase enzymes, such as histone deacetylases (HDACs) and sirtuins, the modification can be removed, thereby allowing for controlled, dynamic regulation of gene expression.

This control over HAT activity is disrupted in disease instances, such as cancer, where abnormal histone acetylation profiles have been shown to correlate with disease progression. Current work is being done to identify the specific epigenetic regulators that contribute to these aberrant acetylation profiles and, more importantly, to develop specific inhibitors and activators of these enzymes.

BPS has a large portfolio of acetyltransferase research tools. Our products include active purified enzymesassay kits, and inhibitors & activators. As with all of our products, we perform rigorous QC testing of our acetyltransferase products to ensure that we are producing only high-quality, consistent products.


Related Technologies:

  • Chemiluminescent

Contact us today for more information about our Screening and Profiling Services. Tailor our services to meet your needs: Learn more about our Custom Services.