RSK1, GST-tag Recombinant

Catalog #

40172

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant full-length human RSK1 was expressed in Sf9 insect sells using an N-terminal GST-tag.

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Synonyms

RPS6KA1; HU-1; MAPKAPK1A; S6K-alpha 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

108 kDa

Amino Acids

full length

Specific Activity

210 pmol/min/µg

Genbank #

NM_002953

UniProt #

Q15418

Tag(s)

N-terminal GST-tag

Background

RSK1 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine/threonine kinases. RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. RSK1 encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue (1). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (2).

References

1. Moller, D E. et al: Human rsk isoforms: cloning and characterization of tissue-specific expression. Am. J. Physiol. 266: C351-C359, 1994.
2. Gross, S D. et al: Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90(Rsk). Science 286: 1365-1367, 1999.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Kinase activity was measured using an S6K synthetic peptide substrate (KRRRLASLR) diluted in distilled water at 1.0 mg/ml.

The protein kinase was diluted in a buffer containing 5 mM MOPS, pH 7.2, 2.5 mM β-glycero-phosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 50 ng/μl BSA (Bovine Serum Albumin) and 0.05 mM fresh DTT.

Samples were prepared with 10 μl of diluted protein, 5 μl of substrate (1 mg/ml), and 5 μl of distilled water. The blank was determined from a “no substrate” sample. The reaction was initiated by addition of 5 μl of an ATP mix containing 1 mCi [33P]-ATP and 0.25 mM ATP, prepared in 6 ml of buffer: 25 mM MOPS, pH 7.2, 12.5 mM β-glycero-phosphate, 25 mM MgCl₂, 5 mM EGTA, 2 mM EDTA and 0.25 mM fresh DTT.

Samples were incubated for 15 minutes at 30°C, and the reaction was terminated by spotting 20 μl of the samples onto P81 phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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40172 Lot 230329

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